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Anti-Ripk3 antibody

Cat.#: 175161

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Special Price 241.9 USD

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Product Information

  • Product Name
    Anti-Ripk3 antibody
  • Documents
  • Description
    Rabbit polyclonal antibody to Ripk3
  • Tested applications
    WB
  • Species reactivity
    Mouse, Rat
  • Alternative names
    R antibody; Rip3 antibody; AW107945 antibody; 2610528K09Rik antibody
  • Isotype
    IgG
  • Preparation
    This antigen of this antibody was synthetic peptide within mouse rip3 aa 440-486 / 486.
  • Clonality
    Polyclonal
  • Formulation
    Liquid, 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:500-1:1,000

  • Validations

    Fig1:; All lanes: Western blot analysis of RIP3 with anti-RIP3 antibody at 1:1,000 dilution.; Lane 1: Wild-type MEF whole cell lysate (20 µg).; Lane 2: RIP3 knockout MEF whole cell lysate (20 µg).; 175161# was shown to specifically react with RIP3 in wild-type MEF cells. No band was observed when RIP3 knockout sample was tested. Wild-type and RIP3 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody ( 1/1,000) and Loading control antibody (Rabbit anti-β-actin, R1207-1, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

    Fig1:; All lanes: Western blot analysis of RIP3 with anti-RIP3 antibody at 1:1,000 dilution.; Lane 1: Wild-type MEF whole cell lysate (20 µg).; Lane 2: RIP3 knockout MEF whole cell lysate (20 µg).; 175161# was shown to specifically react with RIP3 in wild-type MEF cells. No band was observed when RIP3 knockout sample was tested. Wild-type and RIP3 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody ( 1/1,000) and Loading control antibody (Rabbit anti-β-actin, R1207-1, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

  • Background
    Q9QZL0
  • References
    • Rebsamen M. et al, DAI/ZBP1 recruits RIP1 and RIP3 through RIP homotypic interaction motifs to activate NF-kappaB. EMBO Rep. 10:916-922(2009).
    • Kaiser W.J. et al, Toll-like receptor 3-mediated necrosis via TRIF, RIP3, and MLKL. J. Biol. Chem. 288:31268-31279(2013).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"