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Product Name
Anti-RCA antibody
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Description
Rabbit polyclonal antibody to RCA
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Tested applications
WB, IHC-P, IF
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Species reactivity
Arabidopsis thaliana, Nicotiana tabacum, Oryza sativa, Zea mays, Spinacia oleracea, Morus alba
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Alternative names
rubisco activase antibody; RUBISCO ACTIVASE antibody; T5I7.18 antibody
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Isotype
IgG
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Preparation
This antigen of this antibody was synthetic peptide within arabidopsis thaliana rubisco activase aa 190-233.
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Clonality
Polyclonal
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Formulation
Liquid, 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
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Applications
WB: 1:10,000-1:50,000
IHC-P: 1:50-1:200
IF: 1:50-1:200
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Validations
Fig1: Western blot analysis on different tissue lysates using anti-Rubisco activase rabbit polyclonal antibody.; Positive control:; Lane 1: Mulberry leaf; Lane 2: Rice; Lane 3: A. thaliana; Lane 4: Tabacum
Fig2: Immunohistochemical analysis of paraffin-embedded A. thaliana using anti-Rubisco activase rabbit polyclonal antibody. .
Fig3: Immunofluorescence staining of paraffin- embedded A. thaliana using anti-Rubisco activase rabbit polyclonal antibody. .
- Background
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References
- Jurczyk et al. Evidence for alternative splicing mechanisms in meadow fescue (Festuca pratensis) and perennial ryegrass (Lolium perenne) Rubisco activase gene. J Plant Physiol 176:61-64 (2015).
- Yin et al. Characterization of Rubisco activase genes in maize: an α-isoform gene functions alongside a β-isoform gene. Plant Physiol 164(4):2096-106 (2014).
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