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Product Name
Anti-POU2F1 antibody
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Description
Rabbit polyclonal to POU2F1
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Tested applications
WB, IF, ICC/IF
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Species reactivity
Human POU2F1
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Alternative names
42278 antibody; OTF1 antibody; oct-1B antibody; 42278 antibody; Otf1 antibody; NF-A1 antibody; Otf-1 antibody; 2810482H01Rik antibody
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the human POU2F1, and purified by antigen affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/ml
ICC/IF: 0.5-1.5 μg/ml
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Validations
POU2F1 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
POU2F1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunofluorescence
Immunofluorescence staining of POU2F1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human POU2F1 polyclonal antibody (1 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to nucleus.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"