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Product Name
Anti-PNP antibody
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Description
Rabbit polyclonal to PNP
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Tested applications
ELISA, WB, IP
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Species reactivity
Human PNP
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Alternative names
PUNP antibody; PRO1837 antibody; Np antibody; Np-1 antibody; Np-2 antibody; Pnp1 antibody; AL024301 antibody; AU015798 antibody; AL024301 antibody; AU015798 antibody; FLJ94043 antibody; FLJ97288 antibody; MGC117396 antibody; MGC125915 antibody; MGC125916 antibody; NP antibody; Np-1 antibody; Pnp antibody; Pnp1 antibody; PRO1837 antibody; PUNP antibody; Purine Nucleoside Phosphorylase antibody; Purine Nucleoside Phosphorylase antibody; FLJ97312 antibody; Np antibody; Np-2 antibody; NP antibody; PNP antibody
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human PNP (rh PNP; P00491; Met1-Ser289). PNP specific IgG was purified by Human PNP affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 2-10 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human PNP. The detection limit for Human PNP is < 0.039 ng/well.
IP: 1-4 μg/mg of lysate
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Background
The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate.
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References
- de Azevedo,W.F. et al., 2003, Biochem Biophys Res Commun. 312 (3): 767-72.
- Canduri,F. et al., 2005, Acta Crystallogr D Biol Crystallogr. 61 (Pt 7): 856-62.
- Perera,G.K. et al., 2005,Clin Exp Dermatol. 30 (1):27-9.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"