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Product Name
Anti-PLXNA1 antibody
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Description
Rabbit monoclonal antibody to PLXNA1
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Tested applications
WB, ICC, FC
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Species reactivity
Human, Mouse, Rat
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Alternative names
NOV antibody; NOVP antibody; PLXN1 antibody; PLEXIN-A1 antibody
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Isotype
IgG
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Preparation
This antigen of this antibody was recombinant protein
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Clonality
Monoclonal
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Formulation
Liquid, 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
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Applications
ICC: 1:50-1:200
FC: 1:50-1:100
WB: 1:500
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Validations
Fig1: ICC staining Plexin A1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig2: Flow cytometric analysis of LOVO cells with Plexin A1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
- Background
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References
- Delloye-Bourgeois C et al. PlexinA1 is a new Slit receptor and mediates axon guidance function of Slit C-terminal fragments. Nat Neurosci 18:36-45 (2015).
- Hernandez-Enriquez B et al. Floor plate-derived neuropilin-2 functions as a secreted semaphorin sink to facilitate commissural axon midline crossing. Genes Dev 29:2617-32 (2015).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"