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Anti-PLEC antibody

Cat.#: 176512

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Product Information

  • Product Name
    Anti-PLEC antibody
  • Documents
  • Description
    Rabbit monoclonal antibody to PLEC
  • Tested applications
    WB, IHC-P, IP
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    HD1 antibody; PCN antibody; EBS1 antibody; EBSO antibody; PLTN antibody; EBSMD antibody; EBSND antibody; EBSOG antibody; EBSPA antibody; PLEC1 antibody; LGMD2Q antibody; PLEC1b antibody; LGMDR17 antibody
  • Isotype
    IgG
  • Preparation
    This antigen of this antibody was recombinant protein within c-terminal human plectin.
  • Clonality
    Monoclonal
  • Formulation
    Liquid, 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:1,000

    IHC-P: 1:100-1:500

  • Validations

    Fig1:; Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-Plectin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH; 2; O and PBS, and then probed with the primary antibody ( 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Fig1:; Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-Plectin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH; 2; O and PBS, and then probed with the primary antibody ( 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Fig2:; Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Plectin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH; 2; O and PBS, and then probed with the primary antibody ( 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Fig2:; Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Plectin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH; 2; O and PBS, and then probed with the primary antibody ( 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Background
    Q15149
  • References
    • Kulkarni RM et al. Ron receptor signaling is protective against DSS-induced colitis in mice. Am J Physiol Gastrointest Liver Physiol 306:G1065-74 (2014).
    • Eisenberg JL et al. Plectin-containing, centrally localized focal adhesions exert traction forces in primary lung epithelial cells. J Cell Sci 126:3746-55 (2013).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"