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Product Name
Anti-PHOSPHO1 antibody
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Description
Rabbit polyclonal to PHOSPHO1
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Tested applications
ELISA, WB, IP
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Species reactivity
Human PHOSPHO1
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Alternative names
OTTHUMP00000220008 antibody; OTTHUMP00000220009 antibody; D11Moh36 antibody; OTTHUMP00000220006 antibody; Phospo1 antibody; RP23-151M22.3 antibody; Phospo1 antibody; D11Moh36 antibody
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human PHOSPHO1 (rh PHOSPHO1; Q8TCT1-1; Met1-Cys267). PHOSPHO1 specific IgG was purified by Human PHOSPHO1 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/ml
ELISA:0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human PHOSPHO1. The detection limit for Human PHOSPHO1 is < 0.039 ng/well.
IP: 1-4 μg/mg of lysate
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References
- Stewart AJ, et al. (2003) Comparative modelling of human PHOSPHO1 reveals a new group of phosphatases within the haloacid dehalogenase superfamily. Protein Eng. 16(12): 889-95.
- Camps M, et al. (2000) Dual specificity phosphatases: a gene family for control of MAP kinase function. FASEB J. 14(1): 6-16.
- Barford D. (1996) Molecular mechanisms of the protein serine/threonine phosphatases. Trends Biochem Sci. 21(11): 407-12.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"