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  • Anti-phospho-Smad1 (Ser463/Ser465) Rabbit antibody

Anti-phospho-Smad1 (Ser463/Ser465) Rabbit antibody

Cat.#: 167684

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Product Information

  • Product Name
    Anti-phospho-Smad1 (Ser463/Ser465) Rabbit antibody
  • Documents
  • Description
    phospho-Smad1 (Ser463/Ser465) Rabbit monoclonal antibody
  • Tested applications
    WB, IP
  • Species reactivity
    Human, Mouse
  • Isotype
    IgG
  • Preparation
    Antigen: A synthetic phosphopeptide corresponding to residues surrounding Ser463/Ser465 of human Smad1
  • Clonality
    Monoclonal antibody
  • Formulation
    50nM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/1000

    IP: 1/20

  • Validations

    Western blot detection of phospho-Smad1 (Ser463/Ser465) in A549,U2OS,C6 cell lysates using phospho-Smad1 (Ser463/Ser465) Rabbit mAb(1:1000 diluted).Predicted band size:52kDa.Observed band size:52kDa.

    Western blot detection of phospho-Smad1 (Ser463/Ser465) in A549,U2OS,C6 cell lysates using phospho-Smad1 (Ser463/Ser465) Rabbit mAb(1:1000 diluted).Predicted band size:52kDa.Observed band size:52kDa.

  • Background
    The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"