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Product Name
Anti-Phospho-GAB1 (Tyr627) Rabbit antibody
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Description
Phospho-GAB1 (Tyr627) Rabbit polyclonal antibody
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Tested applications
WB, ICC/IF
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Species reactivity
Human, Mouse
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Isotype
IgG
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Preparation
Antigen: Peptide sequence around phosphorylation site of tyrosine 627 (V-E-Y(p)-L-D) derived from Human Gab1.
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Clonality
Polyclonal
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Formulation
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB:1/500-1/1000ICC:1/100-1/200
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Validations
Western blot detection of Gab1(Phospho-Tyr627) in A549 cells untreated or treated with EGF using Gab1(Phospho-Tyr627) Rabbit pAb (dilution 1:500, upper) or Gab1 Rabbit pAb (310130, dilution 1:1000, lower).Predicted band size:110kDa.Observed band size:110kDa.
Immunofluorescence staining of methanol-fixed Hela cells using Gab1(Phospho-Tyr627) Antibody .
Western blot analysis of extracts from 293 cells untreated or treated with EGF using Gab1 (Phospho-Tyr627) Antibody .
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Background
Swiss-Prot Acc.Q13480.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"