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Product Name
Anti-PARP2 antibody
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Description
Rabbit monoclonal antibody to PARP2
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Tested applications
WB, FC
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Species reactivity
Human, Mouse, Rat
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Alternative names
ARTD2 antibody; ADPRT2 antibody; PARP-2 antibody; ADPRTL2 antibody; ADPRTL3 antibody; pADPRT-2 antibody
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Isotype
IgG
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Preparation
This antigen of this antibody was recombinant protein within the c-terminus of human parp2.
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Clonality
Monoclonal
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Formulation
Liquid, 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
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Applications
WB: 1:500-1:1,000
FC: 1:50-1:100
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Validations
Fig1: Western blot analysis of PARP2 on SiHa and Raji cell lysates using anti-PARP2 at 1/500 dilution.
Fig2: Flow cytometric analysis of SH-SY-5Y cells with PARP2 antibody at 1/50 dilution (Fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; Yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
- Background
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References
- Ame J.-C. et al. PARP-2, a novel mammalian DNA damage-dependent poly(ADP-ribose) polymerase. J. Biol. Chem. 274:17860-17868 (1999).
- Bonfiglio J.J. et al. Serine ADP-ribosylation depends on HPF1. Mol. Cell 0:0-0 (2017).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"