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Product Name
Anti-p27 KIP 1 Rabbit antibody
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Description
p27 KIP 1 Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC, IP
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Species reactivity
Human, Rat
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Alternative names
KIP1; MEN4; CDKN4; MEN1B; P27KIP1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human p27 KIP 1
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/1000
IHC: 1/20
ICC/IF: 1/50
FC: 1/20
IP: 1/20
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Validations
Western blot detection of p27 KIP 1 in 3T3,Hela cell lysates using p27 KIP 1 Rabbit pAb(1:1000 diluted).Predicted band size:22kDa.Observed band size:27kDa.
Immunohistochemistry of p27 KIP 1 in paraffin-embedded Human breast cancer tissue using p27 KIP 1 Rabbit pAb at dilution 1/20
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Background
Swiss-Prot Acc.P46527.Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed:28666995). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
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