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Product Name
Anti-OTUB2 antibody
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Description
Rabbit monoclonal to OTUB2
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Tested applications
ELISA, IF, ICC/IF
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Species reactivity
Human OTUB2
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Alternative names
4930586I02Rik antibody; AI413508 antibody; AW557219 antibody; C14orf137 antibody; FLJ21916 antibody; MGC3102 antibody; OTU2 antibody; OTU2 antibody; OTB2 antibody; OTU2 antibody; C14orf137 antibody; OTB2 antibody; OTU2 antibody; AI413508 antibody; AW557219 antibody; 2010015L18Rik antibody; 4930586I02Rik antibody; 2010015L18Rik antibody; OTB2 antibody; OTB2 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human OTUB2 (rh OTUB2; Q96DC9-1; Met1-His234).
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/ml
This antibody can be used at 0.1-0.2 μg/ml with the appropriate secondary reagents to detect Human OTUB2. The detection limit for Human OTUB2 is approximately 0.00245 ng/well.
ICC/IF: 10-25 μg/mL
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Validations
OTUB2 Antibody, Rabbit MAb
Immunofluorescence staining of Human OTUB2 in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human OTUB2 monoclonal antibody (15 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 549-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to cytoplasm and nucleus.
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Background
Otubain 2 (OTUB2) is a member of DUBs that belong to the ovarian tumour (OTU) superfamily of proteins which consists of a five-stranded β-sheet sandwiched in between a small helical amino-terminal region consisting of α1 and α2, and a large helical region comprised of α3-α10. Like other DUBs, otubain 2 (OTUB2) cleaves proteins precisely at the ubiquitin-protein bond so that ubiquitylation process can be reversed and regulated. Otubain 2 (OTUB2)'s active-site cleft is sterically occluded by a novel loop conformation resulting in an oxyanion hole, which consists uniquely of backbone amides. Furthermore, the residues that orient and stabilize the active-site histidine of otubain 2 (OTUB2) are different from other cysteine proteases. This reorganization of the active-site topology provides a possible explanation for the low turnover and substrate specificity of the otubains.
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References
- Balakirev MY, et al. (2003) Otubains: a new family of cysteine proteases in the ubiquitin pathway. EMBO Rep 4 (5): 517-22.
- Nanao MH. (2004) Crystal structure of human otubain 2. EMBO reports. 5: 783-8.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"