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Anti-Nanog antibody

Cat.#: 175589

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Special Price 241.9 USD

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Product Information

  • Product Name
    Anti-Nanog antibody
  • Documents
  • Description
    Rabbit polyclonal antibody to Nanog
  • Tested applications
    WB, IHC-P, ICC/IF
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    EN antibody; ENK antibody; ecat antibody; ecat4 antibody; 2410002E02Rik antibody
  • Isotype
    IgG
  • Preparation
    This antigen of this antibody was klh conjugated synthetic peptide derived from mouse nanog 101-200/305
  • Clonality
    Polyclonal
  • Formulation
    Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
  • Storage instructions
    Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
  • Applications

    WB:1:500-2000

    IHC-P:1:400-800

    IF:1:100-500

    ICC/IF:1:100-500

  • Validations

    Fig1: Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;; Incubation: Anti-Nanog Polyclonal Antibody, Unconjugated 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

    Fig1: Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;; Incubation: Anti-Nanog Polyclonal Antibody, Unconjugated 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

    Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse ovarian); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Nanog) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

    Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse ovarian); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Nanog) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

    Fig3: Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;; Incubation: Anti-Nanog Polyclonal Antibody, Unconjugated 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

    Fig3: Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;; Incubation: Anti-Nanog Polyclonal Antibody, Unconjugated 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

    Fig4: Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;; Incubation: Anti-Nanog Polyclonal Antibody, Unconjugated 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

    Fig4: Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;; Incubation: Anti-Nanog Polyclonal Antibody, Unconjugated 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

    Fig5: Sample:; A431(Human) Cell Lysate at 30 ug; Primary: Anti-Nanog at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 34 kD; Observed band size: 34 kD

    Fig5: Sample:; A431(Human) Cell Lysate at 30 ug; Primary: Anti-Nanog at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 34 kD; Observed band size: 34 kD

    Fig6: Sample:; NIH/3T3(Mouse) Cell Lysate at 30 ug; Primary: Anti-Nanog at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 34 kD; Observed band size: 34 kD

    Fig6: Sample:; NIH/3T3(Mouse) Cell Lysate at 30 ug; Primary: Anti-Nanog at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 34 kD; Observed band size: 34 kD

  • Background
    Q80Z64

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