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Product Name
Anti-MMP2 (3A8) Mouse antibody
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Description
MMP2 (3A8) Mouse monoclonal antibody
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Tested applications
WB, ICC/IF
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Species reactivity
Human, Mouse
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Alternative names
72 kDa gelatinase;72kD type IV collagenase;CLG 4;CLG 4A;CLG4;CLG4A;Collagenase Type 4 alpha;Collagenase type IV A;Gelatinase A;Gelatinase alpha;Gelatinase neutrophil;Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase;Matrix antibody
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Isotype
IgG1
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Preparation
Antigen: Purified recombinant human MMP-2 protein expressed in E.coli.
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Clonality
Monoclonal
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Formulation
PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
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Validations
Western blot analysis of extracts from Raw264.7 cell lysates using MMP-2 mouse mAb (1:200 diluted). Predicted band size:64,72KDa. Observed band size:72KDa.
Western blot analysis of extracts from CHO-K1 (B) and CHO-K1 transfected by MMP-2 fragment(A) cell lysates using MMP-2 mouse mAb (1:2000 diluted). Predicted band size:30KDa. Observed band size:30KDa.
Immunofluorescent analysis of Hela cells fixed fixed by anhydrous methanol at -20u2103 and using MMP-2 mouse mAb (dilution 1:50). DAPI was used to stain nucleus (blue).
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Background
Swiss-Prot Acc.P08253.Isoform 2: Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"