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Product Name
Anti-Lck (2E5) Mouse antibody
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Description
Lck (2E5) Mouse monoclonal antibody
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Tested applications
WB
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Species reactivity
Human
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Isotype
IgG2a
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Preparation
Antigen: Purified recombinant fragment of human Lck expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Ascitic fluid containing 0.03% sodium azide.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
ELISA: 1/10000
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Validations
Western blot detection of Lck in Jurkat and Ramos cell lysates and using Lck mouse mAb (1:1000 diluted).Predicted band size: 56KDa.Observed band size: 56KDa.
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Background
Swiss-Prot Acc.P06239.Lck (lymphocyte-specific protein tyrosine kinase), with 509-amino acid protein (about 56kDa), belongs to the Src non-receptor tyrosine kinases family. By virtue of common structural motifs, the Src family is composed of nine members in vertebrates, including Src, Yes, Fgr, Frk, Fyn, Lyn, Hck, Lck and Blk. Lck is expressed predominantly in T cells and is localized to the inner surface of the plasma membrane. Lck is activated after T cell stimulation and is required for T-cell proliferation and IL-2 production. Aberrant expression or activation of Lck kinase has been reported in both lymphoid and nonlymphoid malignancies. In addition, inhibition of Lck has been a target to prevent lymphocyte activation and acute rejection.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"