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Anti-Lamin B1 Rabbit antibody

Cat.#: 169084

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Special Price 1354.8 USD

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Product Information

  • Product Name
    Anti-Lamin B1 Rabbit antibody
  • Documents
  • Description
    Lamin B1 Rabbit polyclonal antibody
  • Tested applications
    ICC/IF, WB, IHC-P, ELISA
  • Species reactivity
    Human, Mouse, Rat
  • Isotype
    IgG
  • Preparation
    Antigen: The antiserum was produced against synthesized peptide derived from human Lamin B1. AA range:26-75
  • Clonality
    Polyclonal
  • Formulation
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    IF: 1/50-200

    WB: 1/500 - 1/2000

    IHC: 1/100 - 1/300

    ELISA: 1/40000

  • Validations

    Immunofluorescence analysis of rat-kidney tissue. 1,Lamin B1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

    Immunofluorescence analysis of rat-kidney tissue. 1,Lamin B1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

    Western blot detection of Lamin B1 in T47D,3T3,COS7 and C6 cell lysates using Lamin B1 rabbit pAb (1:20000 diluted).Predicted band size:67kDa.Observed band size:68kDa.

    Western blot detection of Lamin B1 in T47D,3T3,COS7 and C6 cell lysates using Lamin B1 rabbit pAb (1:20000 diluted).Predicted band size:67kDa.Observed band size:68kDa.

    Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,Lamin B1 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

    Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,Lamin B1 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

  • Background
    Swiss-Prot Acc.P20700.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"