Anti-KDM1/LSD1 (1E5) Mouse antibody

Cat.#: 168251

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Product Information

  • Product Name
    Anti-KDM1/LSD1 (1E5) Mouse antibody
  • Documents
  • Description
    KDM1/LSD1 (1E5) Mouse monoclonal antibody
  • Tested applications
    WB, ICC/IF, IP
  • Species reactivity
    Human, Monkey
  • Alternative names
    Amine oxidase (flavin containing) domain 2;AOF2;BHC110;BRAF35 HDAC complex protein BHC110;BRAF35-HDAC complex protein BHC110;FAD binding protein BRAF35 HDAC complex antibody; 110 kDa subunit;Flavin-containing amine oxidase domain-containing protein 2;KDM 1;KDM1;Kd antibody
  • Isotype
    Mouse IgG1
  • Preparation
    Antigen: Purified recombinant human KDM1/LSD1 protein fragments expressed in E.coli.
  • Clonality
    Monoclonal
  • Formulation
    Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/1000

    ;ICC: 1/100

  • Validations

    Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-KDM1/LSD1 mouse mAb (dilution 1:100).

    Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-KDM1/LSD1 mouse mAb (dilution 1:100).

    Western blot detection of KDM1/LSD1 in Hela,HCT116 and COS7 cell lysates using KDM1/LSD1 mouse mAb (1:1000 diluted).Predicted band size:110KDa.Observed band size:110KDa.

    Western blot detection of KDM1/LSD1 in Hela,HCT116 and COS7 cell lysates using KDM1/LSD1 mouse mAb (1:1000 diluted).Predicted band size:110KDa.Observed band size:110KDa.

    Immunoprecipitation analysis of Hela cell lysates using KDM1/LSD1 mouse mAb.

    Immunoprecipitation analysis of Hela cell lysates using KDM1/LSD1 mouse mAb.

  • Background
    Swiss-Prot Acc.O60341.Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"