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Product Name
Anti-ITPR2 antibody
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Description
Rabbit polyclonal antibody to ITPR2
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Tested applications
Dot blot, ICC, FC, IHC-P
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Species reactivity
Human, Mouse, Rat
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Alternative names
ANHD antibody; IP3R2 antibody; CFAP48 antibody; INSP3R2 antibody
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Isotype
Rabbit IgG
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Preparation
This antigen of this antibody was recombinant protein within human itpr2 aa 1100-1450.
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Clonality
Polyclonal
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Formulation
Liquid, 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
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Applications
Dot Blot: 1:2,000-1:5,000
ICC: 1:50-1:100
IHC-P: 1:50 -1:200
FC: 1:50-1:100
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Validations
Fig1: Dot blot analysis of anti-ITPR2 on PVDF. 0-4ug antigens were given in this test. Anti-ITPR2 antibody was diluted with 1/500.
Fig2: ICC staining ITPR2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining ITPR2 in HT-29 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining ITPR2 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.
Fig9: Flow cytometric analysis of HepG2 cells with ITPR2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary
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References
- Shi JL et al. High expression of inositol 1,4,5-trisphosphate receptor, type 2 (ITPR2) as a novel biomarker for worse prognosis in cytogenetically normal acute myeloid leukemia. Oncotarget 6(7):5299-5309(2015).
- Zhang F et al. Genome-wide association study identifies ITPR2 as a susceptibility gene for Kashin-Beck disease in Han Chinese. Arthritis Rheumatol 67(1):176-181(2015).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"