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Product Name
Anti-IL18RAP antibody
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Description
Rabbit polyclonal to IL18RAP
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Tested applications
ELISA, IHC-P
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Species reactivity
Human IL18RAP
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Alternative names
ACPL antibody; CD218 antigen-like family member B antibody; CD218b antibody; CDw218b antibody; IL-18 receptor accessory protein antibody; IL-18RAcP antibody; IL18RB antibody; IL-18R-beta antibody; MGC120589 antibody; AcPL accessory protein-like antibody; ACPL antibody; CD218b antibody; IL-1R7 antibody; IL18RB antibody; CDw218b antibody; IL-1R-7 antibody; IL-18RAcP antibody; IL-1RAcPL antibody; IL-18Rbeta antibody; IL-18R-beta antibody; AcPL antibody; IL-18RAcP antibody; IL-1RAcPL antibody; IL-18Rbeta antibody; IL-18R-beta antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human IL18RAP (rh IL18RAP; NP_003844.1; Met1-Gly357). IL18RAP specific IgG was purified by Human IL18RAP affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human IL18RAP. The detection limit for Human IL18RAP is < 0.039 ng/well.
IHC-P: 0.1-1 μg/mL
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Validations
IL18RAP Antibody, Rabbit PAb, Antigen Affinity Purified
Immunochemical staining of human IL1R7 in human lung with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections).
IL18RAP Antibody, Rabbit PAb, Antigen Affinity Purified
Immunochemical staining of human IL1R7 in human brain with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections).
IL18RAP Antibody, Rabbit PAb, Antigen Affinity Purified
Immunochemical staining of human IL1R7 in human colon carcinoma with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections).
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Background
Interleukin 18 receptor accessory protein, also known as IL18RAP and CDw218b (cluster of differentiation w218b), is an accessory subunit of the heterodimeric receptor for IL18. This protein enhances the IL18 binding activity of IL18R1 (IL1RRP), a ligand binding subunit of IL18 receptor. The coexpression of IL18R1 and this protein is required for the activation of NF-kappaB and MAPK8 (JNK) in response to IL18. IL18RAP is required for the high affinity binding of interleukin 18 (IL-18) to its receptor complex. IL18RAP together with IL18R1 mediates IL-18-dependent activation of NF-kappa-B and JNK. Two putative isoforms of IL18RAP were detected and the ratios and total levels of these isoforms may contribute to the aetiology of coeliac disease. IL18R1 and IL18RAP polymorphisms have been found associated with diseases such as schizophrenia, HSV1 seropositivity and atopic asthma. Analysis of IL18R1 and IL18RAP SNPs in 5 European prospective cohorts suggests that the variability of these genes are unlikely to contribute to modulate the risk of CVD in European populations.
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References
- Zhernakova A, et al.. (2008) Genetic analysis of innate immunity in Crohn's disease and ulcerative colitis identifies two susceptibility loci harboring CARD9 and IL18RAP. Am J Hum Genet. 82(5): 1202-10.
- Grisoni ML, et al.. (2009) Lack of association between polymorphisms of the IL18R1 and IL18RAP genes and cardiovascular risk: the MORGAM Project. BMC Med Genet. 10: 44.
- Koskinen LL, et al.. (2009) Association study of the IL18RAP locus in three European populations with coeliac disease. Hum Mol Genet. 18(6): 1148-55.
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