Anti-IFI30 antibody

Cat.#: 100668

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Product Information

  • Product Name
    Anti-IFI30 antibody
  • Documents
  • Description
    Rabbit polyclonal to IFI30
  • Tested applications
    ELISA, WB
  • Species reactivity
    Human IFI30
  • Alternative names
    gamma-interferon-inducible lysosomal thiol reductase antibody; gamma-interferon-inducible lysosomal thiol reductase antibody; gamma-interferon-inducible protein IP-30 antibody; gamma-interferon-inducible protein IP-30 antibody; GILT antibody; GILT antibody; IFI-30 antibody; interferon gamma-inducible protein 30 preproprotein antibody; IP30 antibody; IP30 antibody; legumaturain antibody; lysosomal thio reductase IP30 antibody; lysosomal thiol reductase IP30 antibody; MGC32056 antibody
  • Immunogen
  • Isotype
    Rabbit IgG
  • Preparation
    Produced in rabbits immunized with purified, recombinant Human IFI30 (rh IFI30; P13284; Met1-Lys243). IFI30 specific IgG was purified by Human IFI30 affinity chromatography.
  • Clonality
    Polyclonal
  • Formulation
    0.2 μm filtered solution in PBS with 5% trehalose
  • Storage instructions
    This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
    Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
  • Applications

    WB: 10-20 μg/mL

    ELISA: 0.1-0.2 μg/mL

    This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human IFI30. The detection limit for Human IFI30 is approximately 0.0049 ng/well.

  • Validations

    IFI30 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot

    IFI30 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot

  • Background
    IFI30 belongs to the GILT family. This family includes the two characterised human gamma-interferon-inducible lysosomal thiol reductase (GILT) sequences: P13284 and Q9UL08. It also contains several other eukaryotic putative proteins with similarity to GILT. The aligned region contains three conserved cysteine residues. In addition, the two GILT sequences possess a C-X(2)-C motif that is shared by some of the other sequences in the family. This motif is thought to be associated with disulphide bond reduction. IFI30 is a lysosomal thiol reductase that can reduce protein disulfide bonds. It facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds. It also facilitates MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or crosspresentation. IFI30 may facilitate the complete unfolding of proteins destined for lysosomal degradation and plays an important role in antigen processing.
  • References
    • Haque MA, et al. (2002) Absence of gamma-Interferon-inducible Lysosomal Thiol Reductase in Melanomas Disrupts T Cell Recognition of Select Immunodominant Epitopes. J Exp Med. 195(10):1267-77.
    • Phan UT, et al. (2000) Gamma-interferon-inducible lysosomal thiol reductase (GILT). Maturation, activity, and mechanism of action. J Biol Chem. 275(34):25907-14.
    • Phan UT, et al. (2001) Multiple species express thiol oxidoreductases related to GILT. Immunogenetics. 53(4):342-6.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"