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Product Name
Anti-Histone H2B (7E2) Mouse antibody
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Description
Histone H2B (7E2) Mouse monoclonal antibody
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Tested applications
WB, IHC-P
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Species reactivity
Human, Mouse, Rat
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Isotype
IgG1
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Preparation
Antigen: Synthetic Peptide of Histone H2B
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Clonality
Monoclonal
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Formulation
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000-3000
IF: 1/200
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Validations
Western blot detection of Histone H2B in Hela and COS7 cell lysates using Histone H2B mouse mAb (1:100000 diluted).Predicted band size:14kDa.Observed band size:14kDa.
Immunohistochemical analysis of paraffin-embedded human breast caricnoma using Histone H2B uff08uff09Mouse mAb diluted at 1:500.
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Background
Swiss-Prot Acc.P33778.Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"