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Product Name
Anti-GREM1 antibody
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Description
Rabbit polyclonal to GREM1
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Tested applications
ELISA, WB, IHC-P
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Species reactivity
Mouse Gremlin 1 / GREM1
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Alternative names
BMP antagonist 1 antibody; BMP antagonist 1 antibody; cell proliferation-inducing gene 2 protein antibody; CKTSF1B1 antibody; Cktsf1b1 antibody; cysteine knot superfamily antibody; cysteine knot superfamily 1 antibody; cysteine knot superfamily 1 antibody; DAN domain family member 2 antibody; DAND2 antibody; down-regulated in Mos-transformed cells protein antibody; down-regulated in Mos-transformed cells protein antibody; down-regulated in v-mos-transformed cells antibody; DRM antibody; Drm antibody; Grem antibody; GREMLIN antibody; gremlin 1 antibody; gremlin 1-like protein antibody; gremlin-1 antibody; homolog antibody; IHG-2 antibody; increased in high glucose protein 2 antibody; increased in high glucose-2 antibody; ld antibody; MGC126660 antibody; PIG2 antibody; proliferation-inducing gene 2 antibody; RP23-178K16.1 antibody; PIG2 antibody; ld antibody; Drm antibody; Grem antibody; Cktsf1b1 antibody; DRM antibody; HMPS antibody; MPSH antibody; CRAC1 antibody; CRCS4 antibody; DAND2 antibody; HMPS1 antibody; IHG-2 antibody; DUP15q antibody; C15DUPq antibody; GREMLIN antibody; CKTSF1B1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Mouse Gremlin 1 / GREM1 (rM Gremlin 1 / GREM1; O70326; Met1-Asp184). Gremlin 1 / GREM1 specific IgG was purified by Mouse Gremlin 1 / GREM1 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse Gremlin 1 / GREM1. The detection limit for Mouse Gremlin 1 / GREM1 is approximately 0.00975 ng/well.
IHC-P: 1-10 μg/mL
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Validations
Gremlin 1 / GREM1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of mouse GREM1 in mosue brain with rabbit polyclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections).
Gremlin 1 / GREM1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of mouse GREM1 in mosue heart with rabbit polyclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections).
Gremlin 1 / GREM1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of mouse GREM1 in mosue intestine with rabbit polyclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections).
Gremlin 1 / GREM1 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
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Background
GREM1 belongs to the DAN family. It contains 1 CTCK (C-terminal cystine knot-like) domain. GREM1 is a cysteine knot-secreted protein and acts as an inhibitor in the TGF beta signaling pathway. It inhibits BMP-2, -4, and -7. Inhibition by grem 1 of BMPs in mice allow the expression of fibroblast growth factors (FGFs) 4 and 8 and Sonic hedgehog (SHH) which are necessary for proper limb development. It interacts with SLIT1 and SLIT2 in a glycosylation-dependent manner. As a cytokine, GREM1 may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. It down-regulates the BMP4 signaling in a dose-dependent manner. It also acts as inhibitor of monocyte chemotaxis. GREM1 is highly expressed in small intestine, fetal brain and colon.
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References
- Dimitrov BI, et al. (2010) Genomic rearrangements of the GREM1-FMN1 locus cause oligosyndactyly, radio-ulnar synostosis, hearing loss, renal defects syndrome and Cenani--Lenz-like non-syndromic oligosyndactyly. J Med Genet. 47(8):569-74.
- Heron M, et al. (2011) Genetic variation in GREM1 is a risk factor for fibrosis in pulmonary sarcoidosis. Tissue Antigens. 77(2):112-7.
- van Vlodrop IJ, et al. (2010) Prognostic significance of Gremlin1 (GREM1) promoter CpG island hypermethylation in clear cell renal cell carcinoma. Am J Pathol. 176(2):575-84.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"