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Product Name
Anti-GPA33 antibody
- Documents
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Description
Rabbit polyclonal to GPA33
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Tested applications
ELISA, WB, IHC-P
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Species reactivity
Mouse GPA33
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Alternative names
A33 antibody; BB116197 antibody; 2010310L10Rik antibody; 2210401D16Rik antibody; 2210401D16Rik antibody; A33 antibody; Gpa33 antibody; GPA33 antibody; MGC129986 antibody; MGC129987 antibody; RP11-102C16.2 antibody; 010310L10Rik antibody; BB116197 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Mouse GPA33 (rM GPA33; NP_067623.1; Met 1-Ile 235). GPA33 specific IgG was purified by Mouse GPA33 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/ml
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse GPA33. The detection limit for Mouse GPA33 is approximately 0.00245 ng/well.
IHC-P: 0.1-2 μg/mL
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Validations
GPA33 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
GPA33 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of mouse GPA33 in mouse small intestine with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
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Background
Cell surface A33 antigen, also known as glycoprotein A33, is a single-pass type I membrane protein which is expressed in normal gastrointestinal epithelium and in 95% of colon cancers. GPA33 contains one Ig-like C2-type (immunoglobulin-like) domain and one Ig-like V-type (immunoglobulin-like) domain. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. Intracellular traffic and recycling to the cell surface appear to play a major role in GPA33 function and to have an influence on its surface density superseding translational regulation. GPA33 has become a promising target of immunologic therapy strategies, but its biologic function and potential role in tumorigenesis are unknown. EpCAM protein and GPA33 mRNA expressions are specific and sensitive markers of Barrett's metaplasia (BM). GPA33 may also play a role in cell-cell recognition and signaling.
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References
- Heath J.K., et al., 1997, Proc. Natl. Acad. Sci. USA. 94:469-74.
- Ritter G., et al., 1997, Biochem. Biophys. Res. Commun. 236:682-6.
- Frey,D. et al., 2008, Cancer Biother Radiopharm 23 (1):65-73.
- Rageul,J. et al., 2009, Int J Cancer 125 (12):2802-9.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"