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Product Name
Anti-GLI3 antibody
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Description
Rabbit monoclonal antibody to GLI3
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Tested applications
WB, ICC
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Species reactivity
Human
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Alternative names
PHS antibody; ACLS antibody; GCPS antibody; PAPA antibody; PAPB antibody; PAP-A antibody; PAPA1 antibody; PPDIV antibody; GLI3FL antibody; GLI3-190 antibody
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Isotype
IgG
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Preparation
This antigen of this antibody was recombinant protein within human gli3 aa 1261-1580 / 1580.
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Clonality
Monoclonal
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Formulation
Liquid, 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
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Applications
WB: 1:500-1:2,000
ICC: 1:50-1:100
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Validations
Fig1:; Western blot analysis of Gli3 on PC-3M cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
- Background
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References
- Wang B et al. Hedgehog-regulated processing of Gli3 produces an anterior/posterior repressor gradient in the developing vertebrate limb. Cell 100:423-434 (2000).
- Koyabu Y et al. Physical and functional interactions between Zic and Gli proteins. J Biol Chem 276:6889-6892 (2001).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"