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Product Name
Anti-GATE-16 / GABARAPL2 antibody
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Description
Rabbit polyclonal to GATE-16 / GABARAPL2
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Tested applications
ELISA, IHC-P
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Species reactivity
Human GATE-16 / GABARAPL2
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Alternative names
ubiquitin associated and SH3 domain containing B antibody; UBASH3B antibody; STS-1 antibody; STS1 antibody; TULA-2 antibody; p70 antibody; Cbl-interacting protein Sts-1 antibody; SH3 domain-containing 70 kDa protein antibody; suppressor of T-cell receptor signaling 1 antibody; nm23-phosphorylated unknown substrate antibody; T-cell ubiquitin ligand 2 antibody; cbl-interacting protein p70 antibody; tyrosine-protein phosphatase STS1/TULA2 antibody; ubiquitin-associated and SH3 domain-containing protein B antibody; ubiquitin associated and SH3 domain containing antibody; B antibody; Ubash3b antibody; 2810457I06Rik antibody; BB125008 antibody; TULA-2 antibody; p70 antibody; STS-1 antibody; T-cell ubiquitin ligand 2 antibody; cbl-interacting protein p70 antibody; suppressor of T-cell receptor signaling 1 antibody; tyrosine-protein phosphatase STS1/TULA2 antibody; ubiquitin-associated and SH3 domain-containing protein B antibody; ATG8 antibody; GEF2 antibody; ATG8C antibody; GEF-2 antibody; GATE16 antibody; GATE-16 antibody; Gef2 antibody; GATE-16 antibody; AI173605 antibody; 0610012F20Rik antibody; 2900019O08Rik antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human GATE-16 / GABARAPL2 (rh GATE-16 / GABARAPL2; P60520; Met1-Phe117). GATE-16 / GABARAPL2 specific IgG was purified by Human GATE-16 / GABARAPL2 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human GATE-16 / GABARAPL2. The detection limit for Human GATE-16 / GABARAPL2 is < 0.039 ng/well.
IHC-P: 0.1-2 μg/mL
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Validations
GATE-16 / GABARAPL2 Antibody, Rabbit PAb, Antigen Affinity Purified
Immunochemical staining of human GABARAPL2 in human brain with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
GATE-16 / GABARAPL2 Antibody, Rabbit PAb, Antigen Affinity Purified
Immunochemical staining of human GABARAPL2 in human prostate with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
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Background
GATE-16, also known as ATG8, belongs to the MAP1 LC3 family. It is expressed at high levels in the brain, heart, prostate, ovary, spleen and skeletal muscle. GATE-16 is expressed at very low levels in lung, thymus and small intestine. GATE-16 is involved in intra-Golgi traffic. It modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1.
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References
- Ewing Rob M, et al. (2007) Large-scale mapping of human protein-protein interactions by mass spectrometry. Mol Syst Biol. 3(1):89.
- Okazaki N, et al. (2000) Interaction of the Unc-51-like kinase and microtubule-associated protein light chain 3 related proteins in the brain: possible role of vesicular transport in axonal elongation. Brain Res Mol Brain Res. 85(1-2):1-12.
- Xin Y, et al. (2001) Cloning, expression patterns, and chromosome localization of three human and two mouse homologues of GABA(A) receptor-associated protein. Genomics. 74 (3):408-13.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"