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Product Name
Anti-Gastric intrinsic factor / GIF antibody
- Documents
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Description
Rabbit polyclonal to Gastric intrinsic factor / GIF
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Tested applications
ELISA, IHC-P
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Species reactivity
Human GIF / Gastric intrinsic factor
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Alternative names
AV073125 antibody; gastric intrinsic factor antibody; IF antibody; IF antibody; IFMH antibody; INF antibody; INF antibody; intrinsic factor antibody; intrinsic factor antibody; TCN3 antibody; IF antibody; INF antibody; IFMH antibody; TCN3 antibody; AV073125 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human GIF / Gastric intrinsic factor (rh GIF / Gastric intrinsic factor; P27352-1; Met1-Tyr417). GIF / Gastric intrinsic factor specific IgG was purified by Human GIF / Gastric intrinsic factor affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human GIF / Gastric intrinsic factor. The detection limit for Human GIF / Gastric intrinsic factor is approximately 0.00975 ng/well.
IHC-P: 0.1-2 μg/mL
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Validations
Gastric intrinsic factor / GIF Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human GIF in human stomach with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to parietal cells.
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Background
Gastric intrinsic factor, also known as GIF, belongs to the of the cobalamin transport protein family. It is a glycoprotein produced by the parietal cells of the stomach. Gastric intrinsic factor plays a key role in the absorption of vitamin B12 on in the small intestine. Vitamin B12 bounds to haptocorrin after entry into the stomach. The resulting complex enters the duodenum, where pancreatic enzymes digest haptocorrin. In the less acidic environment of the small intestine, B12 can then bind to gastric intrinsic factor. This new complex travels to the ileum, where special epithelial cells endocytose them. Inside the cell, B12 dissociates once again and binds to another protein, transcobalamin II. The new complex can exit the epithelial cells to enter the liver.
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References
- Gerdin AK. (2010) The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice. Acta Opthalmologica. 88:925-7.
- AU - Berlin H, et al. (1968) ORAL TREATMENT OF PERNICIOUS ANEMIA WITH HIGH DOSES OF VITAMIN B12 WITHOUT INTRINSIC FACTOR. Acta Medica Scandinavica. 184(1-6):247-58.
- Hewitt JE, et al. (1991) Human gastric intrinsic factor: characterization of cDNA and genomic clones and localization to human chromosome 11. Genomics. 10(2):432-40.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"