Anti-FOXA1 Rabbit antibody

Cat.#: 168942

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Product Information

  • Product Name
    Anti-FOXA1 Rabbit antibody
  • Documents
  • Description
    FOXA1 Rabbit monoclonal antibody
  • Tested applications
    WB, IHC-P, ICC/IF, FC
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    HNF3A; TCF3A antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant protein of human FOXA1
  • Clonality
    Monoclonal
  • Formulation
    50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/1000-1/5000

    IHC: 1/20

    ICC/IF: 1/20

    FC: 1/20

  • Validations

    Western blot detection of FOXA1 in C6 cell lysates using FOXA1 Rabbit mAb(1:1000 diluted).Predicted band size:49kDa.Observed band size:49kDa.

    Western blot detection of FOXA1 in C6 cell lysates using FOXA1 Rabbit mAb(1:1000 diluted).Predicted band size:49kDa.Observed band size:49kDa.

  • Background
    Swiss-Prot Acc.P55317.Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' . Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles . Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promoter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"