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Product Name
Anti-FH/Fumarase (9B1) Mouse antibody
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Description
FH/Fumarase (9B1) Mouse monoclonal antibody
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Tested applications
WB, IHC-P, ICC/IF
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Species reactivity
Human, Mouse, Rat
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Isotype
IgG1
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Preparation
Antigen: Synthetic Peptide of FH
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Clonality
Monoclonal
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Formulation
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/3000
IF: 1/200
IHC: 1/50-300
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Validations
Western blot detection of Fumarase in MCF7,Jurkat and Hela cell lysates using Fumarase mouse mAb (1:3000 diluted).Predicted band size:50kDa.Observed band size:50kDa.
Western blot detection of Fumarase in K562,Ramos,Jurkat,Hela cell lysates using Fumarase (9B1) Mouse mAb(1:1000 diluted).Predicted band size:50KDa.Observed band size:50KDa.
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Background
Swiss-Prot Acc.P07954.Also acts as a tumor suppressor.MiscellaneousThere are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors .
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"