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Product Name
Anti-EPHA2 antibody
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Description
Rabbit polyclonal antibody to EPHA2
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat, Dog, Cow, Monkey
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Alternative names
ECK antibody; CTPA antibody; ARCC2 antibody; CTPP1 antibody; CTRCT6 antibody
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Isotype
IgG
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Preparation
This antigen of this antibody was klh conjugated synthetic peptide derived from human epha2 901-976/976
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Clonality
Polyclonal
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Formulation
Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
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Storage instructions
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
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Applications
WB:1:500-2000
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Validations
Fig1: Sample:; Human colon carcinoma lysates Lysate at 30 ug; Intestine (Mouse) Lysate at 30 ug; Primary: Anti- EphA2/Eph receptor A2 at 1/200 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/3000 dilution; Predicted band size : 105kD; Observed band size : 105kD, 52/55kD
Fig2: Sample:; NIH/3T3(Mouse) Cell Lysate at 30 ug; Primary: Anti-EphA2 at 1/1000 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 105 kD; Observed band size: 115 kD
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"