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Product Name
Anti-DNA-PKcs (8D3) Mouse antibody
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Description
DNA-PKcs (8D3) Mouse monoclonal antibody
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Tested applications
WB, IP, ICC/IF
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Species reactivity
Human
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Alternative names
DNA dependent protein kinase catalytic subuni;DNA PKcs;DNA-dependent protein kinase catalytic subunit;DNA-PK catalytic subunit;DNAPK;DNPK1;hyper radiosensitivity of murine scid mutation antibody; complementing 1;HYRC;HYRC1;p350;p460;PRKDC;PRKDC_HUMAN;protein kinas antibody
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Isotype
IgG2b
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Preparation
Antigen: Purified recombinant human DNA-PKcs protein fragments expressed in E.coli
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Clonality
Monoclonal
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Formulation
Purified mouse monoclonal in PBS(pH 7.4)containing with 0.02% sodium azide 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
;ICC: 1/200;Other 1/1000
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Validations
Western blot detection of DNA-PKcs in K562,Molt-4,A549 and HCT116 cell lysates using DNA-PKcs mouse mAb (1:1000 diluted).Predicted band size:450KDa.Observed band size:450KDa.
Western blot detection of DNA-PKcs in Hela,K562,HL-60 and MOLT-4 cell lysates using DNA-PKcs mouse mAb (1:1000 diluted).Predicted band size:450KDa,Observed band size:450KDa.
Immunocytochemistry stain of Hela using DNA-PKcs mouse mAb (1:200).
Immunoprecipitation analysis of Hela cell lysates using DNA-PKcs mouse mAb.
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Background
Swiss-Prot Acc.P78527.The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"