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Product Name
Anti-DNA Ligase IV Rabbit antibody
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Description
DNA Ligase IV Rabbit monoclonal antibody
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Tested applications
WB, IHC-P, ICC/IF
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Species reactivity
Human
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Alternative names
LIG4S antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant protein of human DNA Ligase IV
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Clonality
Monoclonal
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Formulation
50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
IHC: 1/20
ICC/IF: 1/20
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Validations
Western blot detection of DNA Ligase IV in K562,U251MG,HepG2,MCF7 cell lysates using DNA Ligase IV Rabbit mAb(1:500 diluted).Predicted band size:104kDa.Observed band size:104kDa.
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Background
Swiss-Prot Acc.P49917.Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"