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Product Name
Anti-Cyclin D1 Rabbit antibody
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Description
Cyclin D1 Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, IP
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthesized peptide derived from human Cyclin D1
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Clonality
Polyclonal
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Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000-1/5000
IHC: 1/50-1/200
ICC/IF: 1/50-1/100
IP: 1/50
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Validations
Immunohistochemical analysis of paraffin-embedded human bladder, using Cyclin D1 Antibody.
Western blot analysis of Cyclin D1 expression in (1)MCF-7 cell lysates;(2) LnCaP cell lysates.
Immunofluorescent analysis of MCF-7 cells, using Cyclin D1 Antibody .
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Background
Swiss-Prot Acc.P24385.Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"