-
Product Name
Anti-CXCL1 antibody
- Documents
-
Description
Rabbit polyclonal to CXCL1
-
Tested applications
ELISA, WB, IP
-
Species reactivity
Rat CXCL1 / MGSA / NAP-3
-
Alternative names
KC antibody; MGSA antibody; CXCL1 antibody; CXCL1 antibody; Fsp antibody; FSP antibody; gro antibody; Gro1 antibody; GRO1 antibody; GROa antibody; Mgsa antibody; MGSA-a antibody; N51 antibody; NAP-3 antibody; Scyb1 antibody; SCYB1 antibody; FSP antibody; GRO1 antibody; GROa antibody; MGSA antibody; NAP-3 antibody; SCYB1 antibody; MGSA-a antibody; KC antibody; Fsp antibody; N51 antibody; gro antibody; Gro1 antibody; Mgsa antibody; Scyb1 antibody
- Immunogen
-
Isotype
Rabbit IgG
-
Preparation
Produced in rabbits immunized with purified, recombinant Rat CXCL1 / MGSA / NAP-3 (rh CXCL1 / MGSA / NAP-3; NP_110472.1; Ala25-Lys96). CXCL1 / MGSA / NAP-3 specific IgG was purified by Rat CXCL1 / MGSA / NAP-3 affinity chromatography.
-
Clonality
Polyclonal
-
Formulation
0.2 μm filtered solution in PBS
-
Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 10-20 μg/ml
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Rat CXCL1 / MGSA / NAP-3. The detection limit for Rat CXCL1 / MGSA / NAP-3 is < 0.039 ng/well.
IP: 1-4 μg/mg of lysate
-
Validations
CXCL1 / MGSA / NAP-3 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
CXCL1 / MGSA / NAP-3 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
-
Background
The Chemokine (C-X-C motif) Ligand 1, CXCL1, is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GRO?, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-a). CXCL1 already known to be important in osteoarthritis (OA), as a novel target gene of transcription factor AP-2? in chondrocytes and support the important role of AP-2? in cartilage. CXCL1 is a potent neutrophil chemoattractant with recognized roles in angiogenesis and inflammation. CXCL1 is a novel immediate PTH/PTHrP-responsive gene. CXCL1 may act as a chemoattractant for osteoclast precursors. CXCL1 may also have important pro-nociceptive effects via its direct actions on sensory neurons, and may induce long-term changes that involve protein synthesis. CXCL1 plays a critical nonredundant role in the development of experimental Lyme arthritis and carditis via CXCR2-mediated recruitment of neutrophils into the site of infection. CXCL1 functions through CXCR2 to transactivate the EGFR by proteolytic cleavage of HB-EGF, leading to activation of MAPK signalling and increased proliferation of epithelial ovarian cancer (EOC) cells. It might limit tumor growth by reinforcing senescence early in tumorigenesis. Thus, CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation, wound healing, and tumorigenesis.
-
References
- Wang JG, et al. (2008) The chemokine CXCL1/growth related oncogene increases sodium currents and neuronal excitability in small diameter sensory neurons. Mol Pain. 4: 38.
- Acosta JC, et al. (2009) A role for CXCR2 in senescence, but what about in cancer? Cancer Res. 69(6): 2167-70.
- Onan D, et al. (2009) The chemokine Cxcl1 is a novel target gene of parathyroid hormone (PTH)/PTH-related protein in committed osteoblasts. Endocrinology. 150(5): 2244-53.
- Ritzman AM, et al. (2010) The chemokine receptor CXCR2 ligand KC (CXCL1) mediates neutrophil recruitment and is critical for development of experimental Lyme arthritis and carditis. Infect Immun. 78(11): 4593-600.
- Bolitho C, et al. (2010) The chemokine CXCL1 induces proliferation in epithelial ovarian cancer cells by transactivation of the epidermal growth factor receptor. Endocr Relat Cancer. 17(4): 929-40.
- Wenke AK, et al. (2011) The transcription factor AP-2? regulates CXCL1 during cartilage development and in osteoarthritis. Osteoarthritis Cartilage. 19(2): 206-12.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"