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Anti-Chk1 (6C5) Mouse antibody

Cat.#: 168441

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Special Price 441.3 USD

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Product Information

  • Product Name
    Anti-Chk1 (6C5) Mouse antibody
  • Documents
  • Description
    Chk1 (6C5) Mouse monoclonal antibody
  • Tested applications
    WB
  • Species reactivity
    Human, Mouse
  • Isotype
    Mouse IgG1
  • Preparation
    Antigen: Purified recombinant fragment of CHK1 expressed in E. Coli.
  • Clonality
    Monoclonal
  • Formulation
    Ascitic fluid containing 0.03% sodium azide.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/500 - 1/2000

    ELISA: 1/10000

  • Validations

    Western blot analysis of extracts from Hela cells, untreated or treated with UV, using Chk1 (Phospho-Ser280) Rabbit pAb (310037,1:500 diluted,upper) or CHK1 Mouse mAb (168441,1:500 diluted,lower).

    Western blot analysis of extracts from Hela cells, untreated or treated with UV, using Chk1 (Phospho-Ser280) Rabbit pAb (310037,1:500 diluted,upper) or CHK1 Mouse mAb (168441,1:500 diluted,lower).

    Western blot analysis using CHK1 mouse mAb against A431 (1), Hela (2), NIH/3T3 (3) and K562 (4) cell lysate.

    Western blot analysis using CHK1 mouse mAb against A431 (1), Hela (2), NIH/3T3 (3) and K562 (4) cell lysate.

  • Background
    Swiss-Prot Acc.O14757.CHK1, also known as CHEK1, it is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Cdc25, when phosphorylated on serine 216 by chk1 becomes bound by an adaptor protein in the cytoplasm. Therefore it is inhibited from removing the inhibiting phosphate from MPF (mitotic/maturation promoting factor) added by Wee1. Consequently, a cell is prevented from entering mitosis. Chk1 can also phosphorylate p53 at Ser20 in vitro.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"