Anti-Cdk4 (3F9) Mouse antibody
Cdk4 (3F9) Mouse monoclonal antibody
Human, Mouse, Rat
Cdk 4;cdk4;CDK4 protein;CDK4_HUMAN;Cell division kinase 4;Cell division protein kinase 4;CMM 3;CMM3;Crk3;Cyclin dependent kinase 4;Cyclin-dependent kinase 4;Melanoma cutaneous malignant 3;MGC14458;p34 cdk4;PSK J3;PSK-J3. antibody
Antigen: Purified recombinant human CDK4 protein fragments expressed in E.coli.
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4 150 mM NaCl) with 0.02% sodium azide 50% glycerol
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
WB: 1/1000;Other 1/1000
Western blot detection of CDK4 in Hela,Lncap and F9 cell lysates using CDK4 mouse mAb (1:1000 diluted).Predicted band size:34KDa.Observed band size:33KDa.
Western blot detection of CDK4 in Hela,C6 and F9 cell lysates using CDK4 mouse mAb (1:1000 diluted).Predicted band size:34KDa.Observed band size:34KDa.
Swiss-Prot Acc.P11802.Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
- Metformin inhibits cell proliferation in SKM-1 cells via AMPK-mediated cell cycle arrest
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