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  • Anti-CDK1 (3H7) Mouse antibody
  • Anti-CDK1 (3H7) Mouse antibody
  • Anti-CDK1 (3H7) Mouse antibody
  • Anti-CDK1 (3H7) Mouse antibody

Anti-CDK1 (3H7) Mouse antibody

Cat.#: 168291

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Special Price 441.3 USD

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Product Information

  • Product Name
    Anti-CDK1 (3H7) Mouse antibody
  • Documents
  • Description
    CDK1 (3H7) Mouse monoclonal antibody
  • Tested applications
    WB, ICC/IF, FC, IP
  • Species reactivity
    Human
  • Isotype
    Mouse IgG1
  • Preparation
    Antigen: Purified recombinant fragment of CDC2 expressed in E. Coli.
  • Clonality
    Monoclonal antibody
  • Formulation
    Ascitic fluid containing 0.03% sodium azide.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/500 - 1/2000

    ICC: 1/200 - 1/1000

    FC: 1/200 - 1/400

    ELISA: 1/10000

  • Validations

    Flow cytometric analysis of PC-2 cells using CDC2 mouse mAb (green) and negative control (purple).

    Flow cytometric analysis of PC-2 cells using CDC2 mouse mAb (green) and negative control (purple).

    Western blot detection of cdc2 in MCF7,COS7,Jurkat and Hela,3T3 cell lysates using cdc2 mouse mAb (1:1000 diluted).Predicted band size:34KDa.Observed band size:34KDa.

    Western blot detection of cdc2 in MCF7,COS7,Jurkat and Hela,3T3 cell lysates using cdc2 mouse mAb (1:1000 diluted).Predicted band size:34KDa.Observed band size:34KDa.

    Immunofluorescence analysis of Hela cells using CDC2 mouse mAb (green). Red

    Immunofluorescence analysis of Hela cells using CDC2 mouse mAb (green). Red

    Immunoprecipitation analysis of Hela cell lysates using cdc2 mouse mAb.

    Immunoprecipitation analysis of Hela cell lysates using cdc2 mouse mAb.

  • Background
    Swiss-Prot Acc.P06493.The cell division control protein cdc2, also known as cyclin-dependent kinase 1 (Cdk1) or p34/cdk1, plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S-phase and mitosis. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase. The isoform CDC2deltaT is found in breast cancer tissues. Furthermore, cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"