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Product Name
Anti-CDCP1 antibody
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Description
Rabbit polyclonal to CDCP1
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Tested applications
ELISA, IHC-P
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Species reactivity
Mouse CDCP1
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Alternative names
9030022E12Rik antibody; AA409659 antibody; CD318 antibody; CUB domain-containing protein 1 antibody; CUB domain-containing protein 1 antibody; E030027H19Rik antibody; membrane glycoprotein gp140 antibody; membrane glycoprotein gp140 antibody; SIMA135 antibody; subtractive immunization M plus HEp3-associated 135 kDa protein antibody; transmembrane and associated with src kinases antibody; transmembrane and associated with src kinases antibody; TRASK antibody; UNQ2486/PRO5773 antibody; CD318 antibody; TRASK antibody; SIMA135 antibody; AA409659 antibody; 9030022E12Rik antibody; E030027H19Rik antibody
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Mouse CDCP1 (rM CDCP1; Q5U462; Met1-Leu666). CDCP1 specific IgG was purified by Mouse CDCP1 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse CDCP1. The detection limit for Mouse CDCP1 is approximately 0.00975 ng/well.
IHC-P: 0.1-2 μg/mL
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Background
CDCP1 contains three extracellular CUB domains. It is a putative stem cell marker that is highly expressed in some human cancer cells and in both, typical and atypical (cancerous) colons. It interacts with CDH2/N-cadherin, CDH3/P-cadherin, SDC1/syndecan-1, SDC4/syndecan-4 and the serine protease ST14/MT-SP1. It also interacts with SRC and PRKCG/protein kinase C gamma. CDCP1 is taken as a key regulator of EGF/EGFR-induced cell migration. It has been shown that signaling via EGF/EGFR induces migration of ovarian cancer Caov3 and OVCA420 cells with concomitant up-regulation of CDCP1 mRNA and protein. Consistent with a role in cell migration CDCP1 relocates from cell-cell junctions to punctate structures on filopodia after activation of EGFR. It may be involved in cell adhesion and cell matrix association. It also may play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. It has been taken as a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets.
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References
- Conze T, et al.. (2003) CDCP1 is a novel marker for hematopoietic stem cells. Ann N Y Acad Sci. 996 (1): 222-6.
- Hooper JD, et al. (2003) Subtractive immunization using highly metastatic human tumor cells identifies SIMA135/CDCP1, a 135 kDa cell surface phosphorylated glycoprotein antigen. Oncogene. 22(12): 1783-94.
- Scherl-Mostageer M, et al. (2001) Identification of a novel gene, CDCP1, overexpressed in human colorectal cancer. Oncogene. 20(32):4402-8.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"