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Product Name
Anti-CDC42 Rabbit antibody
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Description
CDC42 Rabbit monoclonal antibody
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Tested applications
FC, ICC/IF, WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
TKS; G25K; CDC42Hs antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant protein of human CDC42
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Clonality
Monoclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/2000-1/10000
WB: 1/2000-1/10000
IHC: 1/20
ICC/IF: 1/20
FC: 1/50
IP: 1/20
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Validations
Western blot detection of CDC42 in C6,Hela cell lysates using CDC42 Rabbit mAb(1:1000 diluted).Predicted band size:21kDa.Observed band size:21kDa.
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Background
Swiss-Prot Acc.P60953.Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Plays a role in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. Mediates CDC42-dependent cell migration. Required for DOCK10-mediated spine formation in Purkinje cells and hippocampal neurons. Facilitates filopodia formation upon DOCK11-activation . Also plays a role in phagocytosis through organization of the F-actin cytoskeleton associated with forming phagocytic cups.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"