Anti-CD63 (C-term) Rabbit antibody

All lanes: Anti-CD63 Antibody (C-term) at 1:2000 dilutionnLane 1: A2058 whole cell lysatesnLane 2: A375 whole cell lysatesnLane 3: HL-60 whole cell lysatesnLane 4: THP-1 whole cell lysatesnLane 5: U-87 MG whole cell lysatesnnLysates/proteins at 20 u03bcg per lane. nnSecondarynGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionnnPredicted band size: 25 kDannBlocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing HL-60 cells stained with 169125 (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-spe

AP5333B staining CD63 in human melanoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval

CD63 Antibody (C-term) (Cat. #169125) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CD63 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

All lanes: Anti-CD63 Antibody (C-term) at 1:1000 dilutionnLane 1: A2058 whole cell lysatesnLane 2: MDA-MB-453 whole cell lysatesnnLysates/proteins at 20 u03bcg per lane. nnSecondarynGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionnnPredicted band size: 26 kDannBlocking/Dilution buffer: 5% NFDM/TBST.

Anti-CD63 Antibody (C-term)at 1:2000 dilution + human plasma lysatesnnLysates/proteins at 20 u03bcg per lane. nnSecondarynGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionnnPredicted band size: 25 kDannBlocking/Dilution buffer: 5% NFDM/TBST.