Anti-CD247/CD3-ZETA antibody

Cat.#: 100998

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Product Information

  • Product Name
    Anti-CD247/CD3-ZETA antibody
  • Documents
  • Description
    Rabbit polyclonal to CD247/CD3-ZETA
  • Tested applications
  • Species reactivity
    Human CD247 / CD3-ZETA
  • Alternative names
    4930549J05Rik antibody; A430104F18Rik antibody; AW552088 antibody; CD247 antigen antibody; Cd3 antibody; CD3 antigen antibody; Cd3-eta antibody; Cd3h antibody; CD3H antibody; CD3Q antibody; Cd3z antibody; CD3Z antibody; CD3Z antigen antibody; Cd3zeta antibody; Cd3-zeta antibody; CD3-ZETA antibody; CD3zeta chain antibody; CD3-zeta/eta antibody; RP11-104L21.1 antibody; T3z antibody; T3Z antibody; T-cell antigen receptor complex antibody; T-cell receptor T3 eta chain antibody; T-cell receptor T3 zeta chain antibody; T-cell surface glycoprotein CD3 zeta chain antibody; T-cell surface glycoprotein CD3 zeta chain antibody; TCR zeta chain antibody; Tcrk antibody; TCRZ antibody; Tcrz antibody; zeta polypeptide antibody; zeta polypeptide (TiT3 complex) antibody; zeta subunit antibody; zeta subunit of CD3 antibody; T3Z antibody; CD3H antibody; CD3Q antibody; CD3Z antibody; TCRZ antibody; IMD25 antibody; CD3-ZETA antibody; T3z antibody; Cd3h antibody; Cd3z antibody; Tcrk antibody; Tcrz antibody; Cd3-eta antibody; Cd3zeta antibody; AW552088 antibody; Cd3-zeta antibody; 4930549J05Rik antibody; A430104F18Rik antibody; CD3 antibody
  • Immunogen
  • Isotype
    Rabbit IgG
  • Preparation
    Produced in rabbits immunized with purified, recombinant Human CD247 / CD3-ZETA (rh CD247 / CD3-ZETA; P20963-1; Arg52-Arg164). CD247 / CD3-ZETA specific IgG was purified by Human CD247 / CD3-ZETA affinity chromatography.
  • Clonality
  • Formulation
    0.2 μm filtered solution in PBS
  • Storage instructions
    This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
    Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
  • Applications

    WB: 2-10 μg/ml

    ELISA: 0.1-0.2 μg/ml

    This antibody can be used at 0.1-0.2 μg/ml with the appropriate secondary reagents to detect Human CD247 / CD3-ZETA. The detection limit for Human CD247 / CD3-ZETA is approximately < 0.039 ng/well.

    IP: 0.5-2 μg/mg of lysate

  • Validations

    CD247 / CD3-ZETA Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot

    CD247 / CD3-ZETA Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot

    CD247 / CD3-ZETA Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation

    CD247 / CD3-ZETA Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation

    Immunochemical staining of human GALE in human lung cancer with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).

  • Background
    CD247, also known as CD3-ZETA, belongs to the CD3Z/FCER1G family. It contains 3 ITAM domains. As a -cell receptor zeta, CD247 forms the T-cell receptor-CD3 complex together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and –epsilon. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for CD247 gene. Defects in CD247 can cause immunodeficiency due to defect in CD3-zeta. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens. CD247 may play a role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
  • References
    • 1. Radstake TR, et al. (2010) Genome-wide association study of systemic sclerosis identifies CD247 as a new susceptibility locus. Nat Genet. 42(5):426-9.
    • 2. Dieud P, et al. (2011) Independent replication establishes the CD247 gene as a genetic systemic sclerosis susceptibility factor. Ann Rheum Dis. 70(9):1695-6.
    • 3. Li R, et al. (2012) Association of CD247 with systemic lupus erythematosus in Asian populations. Lupus. 21(1):75-83.