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Product Name
Anti-CD13 / ANPEP antibody
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Description
Rabbit polyclonal to CD13 / ANPEP
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Tested applications
ELISA, IHC-P
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Species reactivity
Mouse ANPEP / APN / CD13
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Alternative names
ANPEP antibody; p150 antibody; APN antibody; CD13 antibody; Cd13 antibody; GP150 antibody; Lap1 antibody; Lap-1 antibody; P150 antibody; PEPN antibody; APN antibody; P150 antibody; p150 antibody; APN antibody; CD13 antibody; LAP1 antibody; PEPN antibody; GP150 antibody; AP-M antibody; AP-N antibody; Cd13 antibody; APN antibody; LAP1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Mouse ANPEP metalloprotease domain (rM ANPEP; Lys 66-Ser 966; NP_032512.2). ANPEP specific IgG was purified by mouse ANPEP affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse ANPEP. The detection limit for Mouse ANPEP is approximately 0.00245 ng/well.
IHC-P: 0.1-2 μg/mL
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Validations
CD13 / ANPEP Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of mouse ANPEP in mouse kidney with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections).
CD13 / ANPEP Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of mouse ANPEP in mouse intestine with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to striated border.
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Background
Aminopeptidase N (ANPEP or APN), also known as CD13, is a cell-surface metalloprotease located in the small-intestinal and renal microvillar membrane, as well as other plasma membranes. It belongs to the peptidase M1 family. CD13 plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases and is involved in the metabolism of regulatory peptides by diverse cell types. CD13/APN is a potent regulator of angiogenesis which is essential for tumor invasion and metastasis, and its transcription in activated endothelial cells is induced by angiogenic growth factors via the RAS/MAPK pathway. In addition, this enzyme has been shown to participate in antigen processing and presentation, and accordingly, defects in this gene appear to be a cause of various types of leukemia or lymphoma and carcinomas.
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References
- Pasqualini R, et al. (2000) Aminopeptidase N is a receptor for tumor-homing peptides and a target for inhibiting angiogenesis.Cancer Res. 60(3): 722-7.
- Mabjeesh SJ, et al. (2005) Aminopeptidase N gene expression and abundance in caprine mammary gland is influenced by circulating plasma peptide. J Dairy Sci. 88(6): 2055-64.
- Dunkel B, et al. (2009) Neutrophil and platelet activation in equine recurrent airway obstruction is associated with increased neutrophil CD13 expression, but not platelet CD41/61 and CD62P or neutrophil-platelet aggregate formation. Vet Immunol Immunopathol. 131(1-2): 25-32.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"