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  • Anti-cas9 antibody

Anti-cas9 antibody

Cat.#: 176553

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Special Price 241.9 USD

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Product Information

  • Product Name
    Anti-cas9 antibody
  • Documents
  • Description
    Rabbit monoclonal antibody to cas9
  • Tested applications
    WB, ICC/IF, IHC-P, FC
  • Species reactivity
    Streptococcus pyogenes
  • Isotype
    IgG
  • Preparation
    This antigen of this antibody was recombinant protein within streptococcus pyogenes cas9 aa 740-980.
  • Clonality
    Monoclonal
  • Formulation
    Liquid, 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:1,000-1:5,000

    ICC/IF: 1:50-1:200

    IHC-P: 1:50-1:200

    FC: 1:50-1:100

  • Validations

    Fig1:; Western blot analysis of CRISPR-Cas9 SP on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.; Positive control:; Lane 1: 293 cell lysates transfected with CRISPR-Cas9; Lane 2: 293T cell lysates transfected with CRISPR-Cas9

    Fig1:; Western blot analysis of CRISPR-Cas9 SP on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.; Positive control:; Lane 1: 293 cell lysates transfected with CRISPR-Cas9; Lane 2: 293T cell lysates transfected with CRISPR-Cas9

  • Background
  • References
    • Vojta A et al. Repurposing the CRISPR-Cas9 system for targeted DNA methylation. Nucleic Acids Res N/A:N/A (2016).
    • Cinesi C et al. Contracting CAG/CTG repeats using the CRISPR-Cas9 nickase. Nat Commun 7:13272 (2016).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"