Anti-Carboxypeptidase B1/CPB1 antibody

Anti-Carboxypeptidase B1/CPB1 antibody

• DataSheet
• Material Safety Data Sheets (MSDS)

Mouse monoclonal to Carboxypeptidase B1/CPB1

IHC-P

Human Carboxypeptidase B1 / CPB1
No cross-reactivity in ELISA with Human CPB1; Mouse CPB1; Human CPA1; Human CPA2; Human CPE

0910001A18Rik antibody; 1810063F02Rik antibody; 2210008M23Rik antibody; AI504870 antibody; Carboxypeptidase B1 antibody; Carboxypeptidase B1 antibody; CPB antibody; Cpb1 antibody; CPB1 antibody; DKFZp779K1333 antibody; PASP antibody; PCPB antibody; CPB antibody; PASP antibody; PCPB antibody; AI504870 antibody; 0910001A18Rik antibody; 1810063F02Rik antibody; 2210008M23Rik antibody

Mouse IgG1

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Carboxypeptidase B1 / CPB1 (rh Carboxypeptidase B1 / CPB1; NP_001862.2; Met1-Tyr417). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Monoclonal

0.2 μm filtered solution in PBS

This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Human Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 307-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.

• Yamamoto, K.K. et al., 1992, J. Biol. Chem. 267: 2575-2581.
• Pezzilli, R. et al., 1994, Digestion. 55: 73-77.
• Barbosa Pereira, P.J. et al., 2002, J. Mol. Biol. 321: 537-547.