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Product Name
Anti-Carbonic Anhydrase VB antibody
- Documents
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Description
Rabbit polyclonal to Carbonic Anhydrase VB
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Tested applications
ELISA, WB, IP
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Species reactivity
Human Carbonic Anhydrase VB / CA5B
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Alternative names
7330410H16Rik antibody; Ca5b antibody; CA5B antibody; Car5b antibody; carbonate dehydratase VB antibody; carbonate dehydratase VB antibody; carbonic anhydrase 5B antibody; carbonic anhydrase 5B antibody; Carbonic Anhydrase VB antibody; Carbonic Anhydrase VB antibody; CarVb antibody; CAVB antibody; CA-VB antibody; CA-VB antibody; D730005F19Rik antibody; MGC39962 antibody; RP23-391L6.3 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human Carbonic Anhydrase VB / CA5B (rh Carbonic Anhydrase VB / CA5B; Q9Y2D0; Cys34-Pro317). Carbonic Anhydrase VB / CA5B specific IgG was purified by Human Carbonic Anhydrase VB / CA5B affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/ml
ELISA: 0.1-0.2 μg/ml
This antibody can be used at 0.1-0.2 μg/ml with the appropriate secondary reagents to detect Human Carbonic Anhydrase VB / CA5B. The detection limit for Human Carbonic Anhydrase VB / CA5B is approximately 0.039 ng/well.
IP: 1-4 μg/mg of lysate
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Validations
Carbonic Anhydrase VB / CA5B Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
Carbonic Anhydrase VB / CA5B Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
Immunochemical staining of human TROP2 in human placenta with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
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Background
Carbonic anhydrase 5B, also known as carbonate dehydratase VB, carbonic anhydrase VB, CA-VB and CA5B, is a member of the alpha-carbonic anhydrase family. The strongest expression of CA5B / CA-VB is in heart, pancreas, kidney, placenta, lung, and skeletal muscle. It is not expressed in liver. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CAs show extensive diversity in tissue distribution and in their subcellular localization. CA5B / CA-VB is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA5A / CA-VA. CA5B / CA-VB has a wider tissue distribution than CA5A / CA-VA, which is restricted to the liver. The differences in tissue distribution suggest that the two mitochondrial carbonic anhydrases evolved to assume different physiologic roles. CA5A / CA-VA is activated by histamine, L-adrenaline, L- and D-histidine, and L- and D-phenylalanine. It is inhibited by coumarins, sulfonamide derivatives such as acetazolamide and Foscarnet (phosphonoformate trisodium salt). CA5B / CA-VB is inhibited by coumarins, sulfonamide derivatives such as acetazolamide (AZA), saccharin and Foscarnet (phosphonoformate trisodium salt).
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References
- Fujikawa-Adachi K, et al.,1999, J Biol Chem. 274 (30): 21228-33.
- Liao, S.Y. et al., 2003, J. Med. Genet. 40:257 - 262.
- Temperini C.et al., 2006, Chemistry 12: 7057-66.
- Temperini C.et al., 2006, J. Med. Chem. 49: 3019-27.
- Supuran, C. T. et al., 2008, Curr Pharm Des. 14 (7): 601-602.
- Elleuche, S. et al., 2009, Curr Genet. 55 (2): 211-222.
- Maresca, A.et al., 2009, J. Am. Chem. Soc. 131:3057-3062.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"