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Product Name
Anti-BMP4 Rabbit antibody
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Description
BMP4 Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, IP, FC
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Species reactivity
Human
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthesized peptide derived from human BMP4
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Clonality
Polyclonal
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Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500-1/2000
IHC: 1/50-1/200
ICC/IF: 1/50-1/200
IP: 1/50
FC: 1/50
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Validations
Western blot analysis of extracts from THP-1 and Raji cells using BMP4 Rabbit pAb at 1:1000 dilution. Predicted band size: 47kDa. Observed band size: 47kDa.
Immunohistochemical analysis of paraffin-embedded human breast cancer, using BMP4 Antibody.
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Background
Swiss-Prot Acc.P12644.Bone morphogenetic proteins (BMPs) were first identified as molecules that can induce ectopic bone and cartilage formation. BMPs belongs to the TGF-β superfamily, playing many diverse functions during development. BMPs are synthesized as precursor proteins and then processed by cleavage to release the c-terminal mature BMP. BMPs initiate signaling by binding to a receptor complex containing type I and type II serine/threonine receptor kinases that then phosphorylate Smad (mainly Smad1, 5 and 8), resulting the translocation of Smad into the nucleus. BMP was also reported to activate MAPK pathways in some systems.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"