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Anti-Atoh1 antibody

Cat.#: 176636

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Special Price 241.9 USD

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Product Information

  • Product Name
    Anti-Atoh1 antibody
  • Documents
  • Description
    Rabbit monoclonal antibody to Atoh1
  • Tested applications
    WB
  • Species reactivity
    Mouse, Rat
  • Alternative names
    Hath antibody; Math antibody; Hath1 antibody; Math1 antibody; bHLHa antibody; MATH-1 antibody; bHLHa14 antibody
  • Isotype
    IgG
  • Preparation
    This antigen of this antibody was recombinant protein.
  • Clonality
    Monoclonal
  • Formulation
    Liquid, 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:500-1:2,000

  • Validations

    Fig1:; Western blot analysis of MATH1 on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody ( 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

    Fig1:; Western blot analysis of MATH1 on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody ( 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

    Fig2:; Western blot analysis of MATH1 on mouse brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

    Fig2:; Western blot analysis of MATH1 on mouse brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

  • Background
    P48985
  • References
    • Castellino RC. et. al. Atoh1/MATH1 Adds Up to Ciliogenesis for Transducing SHH Signaling in the Cerebellum. Dev Cell. 2019 Jan
    • Zhong C. et. al. Atoh1 and other related key regulators in the development of auditory sensory epithelium in the mammalian inner ear: function and interplay. Dev Biol. 2019 Feb

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"