Anti-ATG10 Rabbit antibody

Cat.#: 167797

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Product Information

  • Product Name
    Anti-ATG10 Rabbit antibody
  • Documents
  • Description
    ATG10 Rabbit monoclonal antibody
  • Tested applications
    WB, IP
  • Species reactivity
    Human
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthetic peptide of human ATG10
  • Clonality
    Monoclonal
  • Formulation
    50nM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/1000

    IP: 1/20

  • Validations

    Western blot detection of ATG10 in Hela,A549,U251 cell lysates using ATG10 Rabbit mAb(1:1000 diluted).Predicted band size:25kDa.Observed band size:25kDa.

    Western blot detection of ATG10 in Hela,A549,U251 cell lysates using ATG10 Rabbit mAb(1:1000 diluted).Predicted band size:25kDa.Observed band size:25kDa.

  • Background
    Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).[supplied by OMIM, Mar 2008]

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"