Anti- antibody

Cat.#: 176621

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Product Information

  • Product Name
    Anti- antibody
  • Documents
  • Description
    Rabbit polyclonal antibody to
  • Tested applications
    IF, IHC-P, ELISA
  • Species reactivity
    Arabidopsis thaliana
  • Alternative names
    adaptor protein-4 mu-adaptin antibody; AP4M antibody; F22K18.250 antibody; F22K18_250 antibody
  • Isotype
    IgG
  • Preparation
    This antigen of this antibody was synthetic peptide within a. thaliana ap-4 complex subunit mu aa 1-50 / 451.
  • Clonality
    Polyclonal
  • Formulation
    Liquid, 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
  • Applications

    IF:1:50-1:100

    IHC-P:1:50-1:200

    ELISA:1:10,000

  • Validations

    Fig1:; Immunofluorescence staining of paraffin- embedded A. thaliana using anti-AP-4 complex subunit mu rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with AP-4 complex subunit mu antibody at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.

    Fig1:; Immunofluorescence staining of paraffin- embedded A. thaliana using anti-AP-4 complex subunit mu rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with AP-4 complex subunit mu antibody at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.

    Fig2:; Immunohistochemical analysis of paraffin-embedded A. thaliana tissue using anti-AP-4 complex subunit mu antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH; 2; O and PBS, and then probed with the primary antibody ( 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Fig2:; Immunohistochemical analysis of paraffin-embedded A. thaliana tissue using anti-AP-4 complex subunit mu antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH; 2; O and PBS, and then probed with the primary antibody ( 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Background
  • References
    • Fuji K. et. al. The Adaptor Complex AP-4 Regulates Vacuolar Protein Sorting at the trans-Golgi Network by Interacting with VACUOLAR SORTING RECEPTOR1. Plant Physiol. 2016 Jan;170(1):211-9.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"