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Product Name
Anti-AKR1B1 antibody
- Documents
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Description
Rabbit polyclonal to AKR1B1
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Tested applications
ELISA, WB, IP
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Species reactivity
Human AKR1B1
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Alternative names
ALDR1 antibody; ADR antibody; ALR2 antibody; AR antibody; MGC1804 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human AKR1B1 (rh AKR1B1; P15121; Met1-Phe316). AKR1B1 specific IgG was purified by Human AKR1B1 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human AKR1B1. The detection limit for Human AKR1B1 is approximately 0.00975 ng/well.
IP: 1-4 μg/mg of lysate
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Validations
AKR1B1 Antibody, Rabbit PAb, Antigen Affinity Purified
AKR1B1 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
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Background
Aldose reductase (AKR1B1) belongs to the aldo/keto reductase superfamily. AKR1B1 is a NADPH-dependent aldo-keto reductase best known as the rate-limiting enzyme of the polyol pathway. Expression of AKR1B1 was the highest in lens and retina. It is the first enzyme in the polyol pathway through which glucose is converted to sorbitol which is important for the function of various organs in the body, and has been implicated in the etiology of diabetic complications. AKR1B1 is quite abundant in the collecting tubule cells and thought to provide protection against hypertonic environment. Some human tissues contain AKR1B1 as well as AKR1B10, a closely related member of the aldo-keto reductase superfamily.
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References
- Huang SP, et al. (2010) Aldo-Keto Reductases in the Eye. Journal of Ophthalmology. 326 (3): 625-36.
- Aida K, et al. (2000) Disruption of Aldose Reductase Gene (Akr1b1) Causes Defect in Urinary Concentrating Ability and Divalent Cation Homeostasis. Biochemical and Biophysical Research Communications.277 (2): 281-6.
- Liao CS, et al. (2009) Regulation of AKR1B1 by thyroid hormone and its receptors. Molecular and Cellular Endocrinology. 307 (1-2): 109-17.
- Baba SP, et al. (2009) Posttranslational glutathiolation of aldose reductase (AKR1B1): A possible mechanism of protein recovery from S-nitrosylation. Chemico-Biological Interactions. 178 (1-3): 250-8.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"