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Product Name
Anti-ADSL / Adenylosuccinate Lyase antibody
- Documents
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Description
Rabbit polyclonal to ADSL / Adenylosuccinate Lyase
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Tested applications
ELISA, WB
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Species reactivity
Human ADSL / Adenylosuccinate Lyase
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Alternative names
ASL antibody; AMPS antibody; ASASE antibody; Adl antibody; Asl antibody; AMPS antibody; ASASE antibody; ASase antibody; ASL antibody; ASL antibody; OTTMUSP00000050230 antibody; OTTMUSP00000050233 antibody; OTTMUSP00000050234 antibody; RP5-1042K10.8 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human ADSL / Adenylosuccinate Lyase (rh ADSL / Adenylosuccinate Lyase; P30566-1; Met1-Leu484). ADSL / Adenylosuccinate Lyase specific IgG was purified by Human ADSL / Adenylosuccinate Lyase affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 2-5 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human ADSL / Adenylosuccinate Lyase. The detection limit for Human ADSL / Adenylosuccinate Lyase is approximately 0.00975 ng/well.
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Validations
ADSL / Adenylosuccinate Lyase Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
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Background
Adenylosuccinate lyase, also known as adenylosuccinase, ADSL or ASL, is an enzyme implicated in the reaction of adenylosuccinat converting to AMP and fumarate as part of the purine nucleotide cycle. The two substates of adenylosuccinate lyase (ADSL) are dephosphorylated derivatives of SAICA ribotide (SAICAR) and adenylosuccinate (S-AMP), which catalyzes an important reaction in the de novo pathway of purine biosynthesis. ADSL catalyzes two distinct reactions in the synthesis of purine nucleotides, both of which involve the _-elimination of fumarate to produce either aminoimidazole carboxamide ribotide from SAICAR or AMP from S-AMP. The Adenylosuccinate lyase deficiency is a rare autosomal recessive metabolic disorder characterized by the present of SAICA riboside and succinyladenosine (S-Ado). ADSL defect in different patients is often caused by different mutations to the enzyme.
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References
- Nassogne M, et al. (2000) Adenylosuccinase deficiency: an unusual cause of early-onset epilepsy associated with acquired microcephaly. Brain and development. 22 (6): 383-6.
- Sivendran S, et al. (2004) Two novel mutant human adenylosuccinate lyases (ASLs) associated with autism and characterization of the equivalent mutant Bacillus subtilis ASL. J Biol Chem. 279 (51): 53789-97.
- Lee TT, et al. (1999) His68 and His141 are critical contributors to the intersubunit catalytic site of adenylosuccinate lyase of Bacillus subtilis. Biochemistry. 38 (1): 22-32.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"